TABLE 1.

Summary of PilN mutants and their phenotypes

PilN mutation	Location	BTH PilO interaction	Twitching motility	Surface pili
ES132-133VA	Core	Yes	Yes	Yes
MR141-142KL	Core	Yes	No	No
EV157-158AD	Core	Yes	No	No
Triple mutation (ESMREV132-133–141-142–157-158VAKLAD)	Core	Yes	No	No
L81K	Coiled coils	No	Yes—reduced	Yes—hyperpiliated
L81A	Coiled coils	Yes	Yes	Yes
L82K	Coiled coils	Yes	Yes	Yes
LD64-65KK	Coiled coils	No	No	Yes—reduced
PilN chimera	TMS	No	No	Yes—reduced