FIG 1.

Overview of the P. aeruginosa PA14 nucleoside transport machinery. (A) Schematic representation of the genomic region surrounding the transporter operon nppA1A2BCD-fabI. The 3,719-bp deletion of the ABC transporter nppBCD operon, as well as the overexpressed 7,475-bp ABC transporter region, is indicated. The subcellular locations of the proteins are indicated by different colors. The black arrow indicates an operon structure. (B) Transmembrane domain analysis of ABC transporter permeases NppB and NppC. The upper line indicates the predicted topology from TOPCONS based on amino acid sequences. Red lines indicate an inner membrane orientation, blue lines indicate an outer membrane orientation, gray boxes indicate transmembrane helices spanning from the inside to the outside, and white boxes indicate transmembrane helices spanning from the outside to the inside. Below the line is a graphic interpretation of the reliability of the prediction for each amino acid.