TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) or genotypea | Reference or source |
|---|---|---|
| Strains | ||
| Pseudomonas aeruginosa | ||
| PA14 | Wild type | 51 |
| PA14 ΔdppBCDF | dppBCDF deletion mutant | 7 |
| PA14 ΔnppBCD | nppBCD deletion mutant | This study |
| PA14 ΔdppBCDF ΔnppBCD | dppBCDF nppBCD deletion mutant | This study |
| PA14 pscC::MAR2×T7 | pscC transposon mutant, Gmr | 52 |
| Escherichia coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 lac [F′ proAB lacIqZDM15 Tn10(Tcr)] | Stratagene |
| ST18 | pro thi hsdR+ Tpr Smr; chromosome::RP4-2 Tc::Mu-Kan::Tn7/λpir ΔhemA | 53 |
| W3110 | F− λ− rph-1 IN(rrnD rrnE) | 54 |
| W3110 ΔyejABEF | yejABEF deletion mutant | This study |
| BW28357 | F− λ− ΔlacZ4787(::rrnB-3) hsdR514 Δ(araD-araB)567 Δ(rhaD-rhaB)568 | 55 |
| P. syringae pv. phaseolicola 6/0 | Wild type from bush bean, producer of phaseolotoxin | 56 |
| Plasmids | ||
| pGEM-T Easy | Apr, high-copy-number cloning vector | Promega |
| pPS858-Eco | Apr Gmr, source of Gmr-GFP-FRT cassette | 57 |
| pEX18Ap | Apr, gene replacement vector | 57 |
| pEX18Ap.nppBCD-ko | Apr Gmr, contains 2.9-kb fusion fragment of PA14_41130, Gmr-GFP-FRT, and PA14_41170 | This study |
| pBBR1MCS-5 | Gmr, broad-host-range cloning vector | 58 |
| pBBR5.npp | Gmr, contains 7.5-kb fragment carrying nppA1A2BCD operon under lac promoter control | This study |
| pFLP2 | Apr, source of FLP recombinase | 57 |
| pKD46 | Apr, lambda Red recombinase expression plasmid | 14 |
| pKD3 | Apr Cmr, source of Cmr cassette flanked by FRT sequences | 14 |
| pUHAB | Apr, pUC19 carrying mccABCDE genes from E. coli R51 on 11.2-kb fragment | 59 |
a
Antibiotic resistance: Apr, ampicillin; Gmr, gentamicin; Kan, kanamycin; Smr, streptomycin, Tc, tetracycline; Tpr, trimethoprim.