FIG 1.
Plating defect of the oxyR mutant. The mutant was validated by genetic complementation in our previous study (29). Cultures at mid-log phase were collected, adjusted to 109 cell/ml (dilution factor 0), and then serially diluted, and 5 μl of each dilution was dropped onto agar plates as indicated. The results shown are from after growth of the wild type plated at the lowest cell density (104 cell/ml, dilution factor 5) became evident. Experiments were performed at least three times, and representative results are presented. (A) Effects of thiourea on the plating defect on LB plates. (B) Results on MS plates.