TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | Host for cloning | Laboratory stock |
| WM3064 | ΔdapA, donor strain for conjugation | W. Metcalf, UIUC |
| S. oneidensis strains | ||
| MR-1 | Wild type | Laboratory stock |
| HG0956-8 | ΔahpCF, derived from MR-1 | 29 |
| HG1070 | ΔkatB, derived from MR-1 | 29 |
| HG1328 | ΔoxyR, derived from MR-1 | 29 |
| HG3CAT | ΔkatB ΔkatG1 ΔkatG2, derived from MR-1 | 29 |
| HGACAT | Δahp ΔkatG1 ΔkatG2, derived from MR-1 | This study |
| HG0956-8-1070 | ΔkatB ΔahpCF, derived from MR-1 | This study |
| HG0976-1070 | ΔkatB Δohr, derived from MR-1 | This study |
| HG1070-2178 | ΔkatB ΔccpA, derived from MR-1 | This study |
| Plasmids | ||
| pHGM01 | Apr Gmr Cmr, att-based suicide vector | 31 |
| pHG101 | Kmr, promoterless broad-host vector | 32 |
| pHGE-Ptac | Kmr, IPTG-inducible expression vector | 33 |
| pHGEI01 | Kmr, integrative lacZ reporter vector | 34 |
| pBBR-Cre | Spr, helper plasmid for antibiotic cassette removal | 56 |
| pHGE-Ptac-ahpCF | Vector for inducible expression of Ahp | This study |
| pHGE-Ptac-katB | Vector for inducible expression of KatB | This study |
| pHGEI01-ryhB | pHGEI01 containing the ryhB promoter | This study |