FIG 3.

DAPG production by P. protegens alters colony morphology in B. subtilis when they are grown in coculture. B. subtilis grown on 1× MSgg agar either alone, next to the DAPG-producing wild-type P. protegens Pf-5, or next to the DAPG-biosynthesis mutant P. protegens Pf-5 phlD after 24, 48, and 72 h. In the cocultures, B. subtilis is shown on the left of each panel. The centers of the initial cell suspensions were placed 0.5 cm away from each other. In the center panel, the black arrowhead points to a region of the colony exhibiting normal fruiting body formation, while the white arrowhead indicates a region where a lack of fruiting bodies leads to smooth colony morphology (see Fig. S4 in the supplemental material). Scale bar, 2 mm.