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Enhanced gene transcription requires intact SpxA1 protein. In vitro transcription reactions were performed with the regulatory regions of ahpC, sodA, or tpx incubated with B. subtilis RNAP without SpxA1 (0), with MBP-tagged SpxA1 (A1), or with MBP-tagged SpxA1G52R (A1G52R). Radiolabeled RNA transcripts were precipitated, applied to 8% urea PAGE, and visualized by exposure to a phosphorimager screen. Representatives of three or more independent reactions are shown.
