. 2015 Jun 3;197(13):2201–2216. doi: 10.1128/JB.00197-15

TABLE 5.

Genetic complementation of mutant P13-7 restores wild-type phenotypic traits

Strain	Insect pathogenesis^a		Swim motility^b	Symbiosis^c	Dye binding^d		Extracellular production^e				Pigment
Strain	LT₅₀	LT₁₀₀	Swim motility^b	Symbiosis^c	EB	MacConkey	DNase	Protease	Hemolysin	Antibiotic	Pigment
NC19 primary (parental)	36	48	48.0 ± 3.3	+	+	+	++	++	+	+	+
HNR0606 (NC19+ pHR1)	33	43	62.2 ± 5.3	+	+	+	++	++	++	+	+
HNR0018 (NC19 + pBAD32)	36	48	52.2 ± 6.3	+	+	+	++	++	+	+	+
P13-7	60	72	22.0 ± 1.1	+	−	−	++	−	−	−	−
HNR1307 (P13-7 + HR1)	39	49	49.5 ± 5.0	+	+	+	++	++	+	+	+
HNR1318 (P13-7+ pBAD32)	61	70	19.0 ± 1.1	+	−	−	++	−	−	−	−

Insect pathogenesis was determined on G. mellonella larvae as described in Materials and Methods. LT₅₀ and LT₁₀₀ are the time (in hours) required to kill 50 and 100% of larvae.

The swim migration assay was carried out after 48 h of incubation at 28°C. Values are average swim ring diameters, with standard deviations. Results are averages of 3 measurements per independent experiment.

Symbiosis was determined by the nematode assay and determined by the development of mature IJs. +, capable of normal nematode development, similar to the parental wild type.

Dye absorption on EB and MacConkey plates were noted at 48 h. A positive result on EB plates was indicated by metallic green colonies, and a negative result was indicated by dull purple colonies. A positive result on MacConkey plates was indicated by bright red colonies and a negative result by colorless or pink colonies.

DNase and protease activities were determined by measuring the sizes (in millimeters) of the halos surrounding the bacterial colonies 24 h after inoculation. ++, strongly positive (2- to 4-mm halo); +, positive (1- to 2-mm halo); −, negative (no halo). Hemolytic activity was determined by clearing zones around the bacterial colonies. ++, enhanced annular hemolysis; +, strong annular hemolysis; −, negative (no hemolysis). Antibiotic production was determined by measuring the sizes (in millimeters) of the halos surrounding the bacterial colonies 1 day after inoculation of the tester bacterium (Micrococcus luteus). +, positive (2- to 5-mm halo); −, negative (no halo).