TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristic(s) | Resistance | Source or reference |
|---|---|---|---|
| S. meliloti strains | |||
| Rm1021 | Wild-type strain SU47 Smr | Sm | F. Ausubel |
| CSS6000 | Rm1021 ΔcbrA::cat | Cm | 46 |
| KBS1001 | Rm1021 carrying plasmid pcpdR1 | Tc | This study |
| KBS1002 | Rm1021 carrying plasmid pcpdR1D53A | Tc | This study |
| KBS1003 | CSS6000 carrying pcpdR1 | Tc | This study |
| KBS1004 | CSS6000 carrying pcpdR1D53A | Tc | This study |
| CSS6005 | Rm1021 carrying plasmid pLAFR2070 | Tc | This study |
| CSS6006 | Rm1021 carrying plasmid pLAFR1 | Tc | This study |
| CSS6002 | CSS6000 carrying pLAFR2070 | Cm Tc | 46 |
| CSS6003 | CSS6000 carrying pLAFR1 | Cm Tc | 46 |
| E. coli strains | |||
| MT616 | Strain MM294 carrying pRK600 Cmr | Cm | T. Finan |
| DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argG) | BRL Corp. | |
| BL21 | ompT hsdSB(rB− mB−) gal dcm (DE3) | Cm | Invitrogen |
| Plasmids | |||
| pLAFR1 | Low-copy-number vector | Tc | F. Ausubel |
| pLAFR2070 | cbrA+ complementation vector | Tc | 20 |
| pDEST17::ctrA | Gateway destination vector containing S. meliloti ctrA | Kn | 46 |
| pcpdR1 | pTH1227 cpdR1 | Tc | 33 |
| pcpdR1D53A | pTH1227 cpdR1D53A | Tc | 33 |