FIG 8.

cAMP levels produced by both the ACs are affected by DGC expression. Intracellular levels of cAMP in ΔcyaA and ΔcyaB mutant strains were measured using an EIA (A). The cAMP levels were measured during expression of PA1120 DGC (pJN1120) or with the vector control (pJN105). Vfr-cAMP transcriptional activity was measured in the same strain by using the mini-CTX::lacP-lacZ transcriptional reporter (B). Data sets represent results of at least two biological experiments with three technical replicates each. All measurements were standardized to the total protein content. Bars indicate standard deviations of the means. Statistical significance (P ≤ 0.001) is indicated with three asterisks.