Figure 2.

Morphological features of AAA-MSCs exposed to hypoxia, nutrient deprivation, dry culture, and/or cold temperature. (A) Typical spindle-shape morphology of confluent AAA-MSCs at passage 3, grown in standard culture conditions; (B) Clusters of AAA-MSCs cultured in no-vented closed flasks using MEM glucose-free at 7 days and (C) at 14 days from seeding, where a cell monolayer is visible; (D,E) AAA-MSCs subjected to media deprivation lost their typical structure, cytoplasmic prolongation and plasma membrane; (F) 7 days from media addition, an amount of survival cells was detected; (G,H) AAA-MSCs after media deprivation and 7 days at 4°C showed a volume contraction and the typical cell shape was lost; (I) after traditional media addition, AAA-MSCs restored their features and reached confluence; (A–G,I: 100x magnification; H: 200x magnification).