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. 2015 Jun 4;3:36. doi: 10.3389/fcell.2015.00036

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Copyright © 2015 Ciavarella, Fittipaldi, Pedrini, Vasuri, Gallitto, Freyrie, Stella, Gostjeva and Pasquinelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxic assay and transcriptional profile of AAA-MSCs exposed to CoCl₂. (A) AAA-MSC viability after 24–48–72 h exposure to CoCl₂ (0-100-500 μM), measured by sulforhodamine B assay. Values are represented as mean ± standard deviation and are representative of at least three independent experiments (^*p < 0.05; ^***p < 0.001; Two-Way ANOVA test, followed by Bonferroni post-hoc test). (B) mRNA expression of NANOG, OCT-4 and SOX-2 after 48–72 h exposure to CoCl₂ (0-100-500 μM), performed by RT-PCR. GAPDH was used as housekeeping gene. Results are representative of three independent experiments.