Skip to main content
. 2015 May 21;56(5):3242–3252. doi: 10.1167/iovs.14-16274

Figure 3.

Figure 3

Autofluorescent granules aggregate into packets and disappear, reducing AF. (A–F) Retinal pigment epithelial cells of AMD eyes sequester LF/MLF granules into dense packets (white arrowhead), in association with a rounding of the cytoskeleton (yellow arrowheads [A]). In late stages, packets of LF/MLF granules are reduced in number diminishing total AF signal further (C–F). Aggregation proceeds concurrently with degranulation (D). (G) Massive enlarged cell, possibly healthy, with hundreds of AF granules and not yet undergoing degranulation. A histologic cross-section of granule aggregations is shown in Supplementary Figure S3. Donors: (A, B, D–F) 83 years, female, incipient AMD; (C, G) 81 years, male, late nonexudative AMD. (A–F) Confocal microscopy, (G) HR-SIM. F-actin labeled with AlexaFluor647-Phalloidin. Scale bars: 20 μm.