Figure 1.

Detecting the expression of 11 selected miRNA in cardiac tissue samples. Total RNA was extracted from tissue samples using Trizol reagent following the manufacturer’s instructions. The expression level of candidate miRNAs was detected by TaqMan miRNA RT Real-Time PCR. Single-stranded cDNA was synthesized by using the TaqMan MicroRNA Reverse Transcription kit and then amplified by using TaqMan Universal PCR Master Mix together with miRNA-specific TaqMan MGB probes. The U6 snRNA was used for normalization. Each sample in each group was measured in triplicate and the experiment was repeated at least 3 times for the detection of miRNAs. The t test was used to analyze the results and P<0.05 was considered statistically significant. * P<0.05.