Figure 3.
Low miR-101 level contributes to high cytokines level in PGN-stimulated THP-1 cell culture supernatant. THP-1 cells were seeded at 5×105 cells/ml in a 24-well plate. At 48 h after miR-101 transfection, cells were stimulated with various doses of PGN (0.01–1 μg/ml) for 24 h. The cell culture supernatants were collected and the concentration of TNF-α (A), IL-1β (B), and IL-6 (C) was determined by ELISA. The results were analyzed by t test and P<0.05 was considered as statistically significant. * P<0.05.