Figure 4.

Low miR-101 level is related to over-expressed cytokines and TLR2 in clinical samples. (A) Serum TNF-α, IL-1β, and IL-6 concentration was analyzed by using an ELISA kit. (B) The mRNA level in cardiac tissue samples was detected by qRT-PCR. (C) TLR2 protein in cardiac tissues was detected using Western blot with β-actin as loading control. (D) TLR2 mRNA level in cardiac tissues was detected by using qRT-PCR. The results were analyzed by t test and P<0.05 was considered as statistically significant. * P<0.05, ** P<0.01.