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. 2015 Apr 15;7(4):670–682.

Figure 1.

Figure 1

Exposure to 50 μM BMS-345541 does not influence the expression of nuclear p65 or cytosolic IκB-α in freshly isolated mdx costal diaphragms. A. Western blots of the nuclear expression of p65 and Oct 1 and the cytosolic expression of IκB-α and GAPDH. Freshly isolated mdx costal diaphragms were exposed to either vehicle (0.1% DMSO) or 50 μM BMS-345541 for 6 hours at room temperature before being flash frozen for subsequent analyses of the expression of p65 and IκB-α in nuclear and cytosolic fractions of the muscle, respectively. B. Densitometry results from nuclear (left - p65/oct1) and cytosolic fractions (right - IκB-alpha/GAPDH) obtained from the vehicle-treated and drug-treated (50 μM BMS-345541) preparations. Note that BMS-345541 produced a slight increase in cytosolic IκB-α and reduction in nuclear p65 that did not reach statistical significance.