Figure 7.

Long term intraperitoneal administration of PHA-408 reduces the nuclear expression of p65 in mdx costal diaphragm. PHA-408 (0.8 mg/kg) or vehicle (HEPES buffered isotonic saline) was administered daily to 1 month old mdx mice for a period of 30 days. Inset shows Western blots obtained from costal hemi-diaphragms of vehicle and drug-treated mice following the treatment period. N refers to the number of mice and costal hemi-diaphragms. Densitometric analyses (bar graph; p65/0ct-1) revealed a significant (*-p < 0.05) 50% reduction in nuclear p65 expression in the mice treated daily with PHA-408.