Fig. A4.

Compilation of different overall methods for calculating electrophysiological properties from the sample of curated articles in the quality control (QC) audit. A–F: pie charts and labels indicate breakdown of electrophysiological calculation methodology and n indicates number of property measurements found in sample. Label “unreported” indicates that no specific methodological description could be found; n = 27 articles quantified in QC subset. A: resting membrane potential (V_rest), label “spontaneously active pseudo-V_rest method” indicates methodology for quantifying V_rest in spontaneously active neurons. B: input resistance (R_input), label “leak method” indicates method for calculating R_input based on leak current. C: membrane time constant (τ_m), label “peeling method to mitigate I_h” indicates method calculating τ_m that corrects for sag current influence, label “C_s⁺” indicates the use of cesium ions in the electrode pipette solution. D: action potential half-width (AP_hw). Labels indicate different protocols for eliciting spikes from which AP_hw is calculated. By definition, all AP_hw measurements have been quantified as AP full-width at half-maximal amplitude, usually from the first evoked AP in train. E: action potential amplitude (AP_amp). Pie charts indicate methodology for quantifying AP_amp (left) or protocol used to elicit action potentials (right). Quantification labels indicate whether AP_amp is defined as the difference between AP threshold and peak or V_rest and AP peak. F: action potential threshold (AP_thr), label “max inflection point” indicates identification of action potential threshold via 2nd derivative of voltage.