Table 3.
Cytokine levels in the sera / supernatants of stimulated-splenocytes and expression of corresponding genes in the spleens of non-pregnant and pregnant immunized mice at different time points
| Category and time after the dose | Serum cytokines (pg/ml) | Gene expression spleen (fold change) | Splenocytes (rNEp-stimulated , pg/ml) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| IL-2 | IL-4 | IFN-γ | IL-2 | IL-4 | IFN-γ | IL-2 | IL-4 | IFN-γ | |
| NPR 24 hrs* | UD | UD | UD | 7.6D | 2.7D | 2.8U | 5 | 5 | 4.7 |
| NPR 2 weeks | UD | UD | UD | 2.5D | 5.7D | 3.1D | 5 | 10 | 30 |
| PI 24 hrs** | 19.4 | UD | 56.6 | 0.7 | 3.7D | 0.9 | 5 | 4.5 | 4.8 |
| PI 2 weeks | 2.7 | UD | 6.2 | 3.2D | 0.9 | 2.9D | 4.5 | 20 | 10 |
| PNI day 8*** | 22.2 | UD | UD | 2.6D | 2.2D | 3.2D | 4.8 | 4.5 | 4.2 |
| PNI day 18 | 4.7 | UD | UD | 4.3D | 4.2D | 3D | 5 | 4.5 | 4.8 |
*indicates non-pregnant immunized mice, **indicates pregnant immunized mice, ***indicates pregnant unimmunized mice. UD = undetectable, D = downregulation, U = upregulation.