Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 21;14:108. doi: 10.1186/s12943-015-0371-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Heubach et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Cell type dependent induction of an aggressive phenotype in VM-CUB1 cells. (a) Phenotypical changes of stably transfected VM-CUB1 and 5637 clones as seen in light microscopy (10x magnification). (b) Immunofluorescence stainings for E-Cadherin (top, green) and Vimentin (bottom, green) to verify EMT induction in VM-CUB1-HOTAIR 20 cells (40 x magnification). Transwell assays were performed to determine changes in migration (c) and invasion (d, e) capacity (**p ≤ 0.001). Data results from one assay with 4 transwells per cell line and 5 visual fields, which were randomly chosen for counting the number of migrated or invaded cells. Results are representative for assays repeated in triplicate. Invaded cells were visualized by H&E staining (d, 10 x magnification).