Figure 12.
Effects of implantation of DFAT cells with TSG-6 siRNA on proteinuria and renal function in mAb 1-22-3-induced glomerulonephritis. Wistar rats were injected with 0.5 mg of mAb 1-22-3 and saline or 106 DFAT cells transfected with 20 nM TSG-6 siRNA or 20 nM control siRNA through the tail vein. Twenty-one days after the neflectomy urine was collected for 24 hours and blood was sampled. Urinary protein excretion (A), and serum levels of BUN (B) and creatinine (Cre) (C) were measured. Data are the mean ± SEM (n = 4). *P <0.05 in the indicated columns. Bar = 50 μm. BUN, blood urea nitrogen; DFAT, dedifferentiated fat; mAb, monoclonal antibody; SEM, standard error of the mean; TSG-6, TNF-stimulated gene-6.