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. 2015 Apr 16;6(1):80. doi: 10.1186/s13287-015-0069-2

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© Maruyama et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of implantation of DFAT cells on mRNA expression of renal injury markers and nephrin in renal cortex in mAb 1-22-3-injected rats. Wistar rats were nephrectomized and injected without mAb 1-22-3 (Control) or with 0.5 mg mAb 1-22-3 through the tail vein seven days after the nephrectomy. Thirty five days after the nephrectomy saline (Saline) or 10⁶ DFAT cells were injected through the renal artery (DFAT ia) or tail vein (DFAT iv). Sixty-three days after the nephrectomy, the left kidney was removed. Real-time quantitative PCR was performed to determine the expression of Kim-1 (A), collagen IV (B), fibronectin (C) and nephrin (D) mRNAs. Data are the mean ± SEM (n = 6). *P <0.05 and **P <0.01 in the indicated columns. DFAT, dedifferentiated fat; Kim-1, kidney injury molecule; mAb, monoclonal antibody; SEM, standard error of the mean.