Figure 2.

Time course of the ‘MiI’ on inhibition of nAChRs currents at room temperature in SCG neurons. (a) The inhibition of 100 µM Nic-induced currents was strongly dependent on prepuff-time of MCh (1 mM) solution. In addition to the MCh-prepuff solution, MCh (1 mM) was also included in the Nic-puffing solutions, except the currents of ‘control’ and ‘recover’. Note that the ‘control’ current is nearly overlapped by the current of 0 s prepuff. MCh was washed out using standard external solution during and after the 150 s break. All traces in this panel were from one neuron. (b) Time course of the MCh effect in (a). The data points of the prepuff effect were well described by an exponential function with time constant of 1.3 s. Prepuff for 6 s was sufficient to get maximum MiI of 81 ± 1% (n = 8). (c) The recovery of nAChRs inhibition induced by MCh (1 mM) was strongly dependent on wash time. All traces in this panel were from another neuron. (d) Statistics of the wash experiments in (c). At 22°C, the data points were well fitted by a two exponential function with time constants of 10.0 s and 88.7 s, respectively (n = 7).