Skip to main content
. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Neoplasma. 2015;62(1):41–52. doi: 10.4149/neo_2015_006

Figure 3.

Figure 3

Fn14 induces expression of integrin α6. A) RT-PCR analysis of mRNA levels of integrin α6 in Fn14 isogenic variants of H460 cells. Integrin α6 (ITGA6) expression was normalized to GAPDH control. The results are obtained from a minimum of three independent experiments. Asterisks indicate: *p<0.05; **p<0.0005 (two-tailed unpaired Student's T-test). B) Immune-precipitation using anti-α3 antibody showing that none of the H460 variants with altered Fn14 expression produced integrand α3 protein. Immune-precipitation using anti-α6 antibody and western blot analysis (using AA6α antibody) of cell lysates of H460 isogenic variants showing increased levels of integrin α6 in cells over-expressing Fn14.