Figure 4.

Expression of Fn14 affects H460 tumor cell migration and invasion. A) High expression levels of Fn14 increased cell migration through uncoated transwells as well as invasion through madrigal coated inserts, while loss of expression decreased migration relative to control cells. The data are shown as pixel intensities of cells invaded through the uncoated insert or madrigals with standard error bars and are the result of at least three independent experiments. Asterisk indicate p< 0.01 (two-tailed unpaired Student's T-test). B) Invasion of H460 cells expressing high levels of Fn14 was significantly decreased in the presence of 5μM anti-MMP-1 antibody while invasion of H460 cells with low Fn14 expression was not f by anti-MMP-1 functional blocking antibody. The data are shown as pixel intensities of cells that invaded through the madrigal with standard error bars and are the result of at least three independent experiments. Asterisk indicate p< 0.01 (two-tailed unpaired Student's T-test) relative to Gig treated cells. C) Fn14 over-expressing cells had made integrin alpha 6-dependent anastomosing structures while Fn14-silnced cells were largely unorganized. The data are shown as representative microscopic pictures from at least three independent experiments.