Figure 3.

Overview of experimental design for mapping small effect mutations affecting the expression of a fluorescent reporter in EMS-induced mutants. This approach is based on the isolation of a large number of random F₁ segregant haploid cells, followed by high-throughput phenotypic selection using FACS, and estimation of allele frequencies genome-wide using next generation sequencing. Note the selection of haploid MATα cells using expression of the RFP reporter linked to MATα locus that is indicated with a red dot. Quantitative differences in the level of YFP expression are indicated by differences in the intensity of yellow background.