Figure 5.

BSA-seq clearly identified a single causative site in three trans-regulatory mutants affecting fluorescence of a reporter gene. Significance of the difference in allele frequency between low fluorescence and high fluorescence bulks is shown as the negative of logarithm of P-value from G-test for mutants YPW89 (A), YPW94 (B), and YPW102 (C). Each bar shows significance for an individual EMS-induced mutation with its genomic position represented on x-axis. Roman numerals indicate each of the 16 S. cerevisiae chromosomes. Insets in (A) and (B) are magnifications of chromosomes harboring causative sites and show linked mutations with significant effects. Horizontal dotted lines represent a significance threshold of α = 0.001.