Table 2. Average genome coverage and number of mutations detected from Illumina sequencing of the two segregant bulks for each of the three mutants.

	Low Bulk		High Bulk		No.
Mutant	Meana	IQR	Meana	IQR	Mutations	G:C ➔ A:T
YPW89	134.1	109−160	111.7	103−126	65	91.0%
YPW94	134.0	107−163	75.3	61−90	73	84.4%
YPW102	91.9	74−109	132.2	121−147	33	84.9%

IQR, interquartile range of genome coverage.

^aMean coverage obtained after read alignment using genomecov tool from BEDTools.