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. 2014 May 15;4(7):1259–1274. doi: 10.1534/g3.114.011080

Figure 3.

Figure 3

Whole genome sequencing of AR116 uncovered a new allele of aux1. (A) Examination of AUX1 in AR116 revealed a G-to-A mutation in the first exon, which causes a Trp43-to-stop mutation. The AR53 causative mutation (aux1-53), identified by recombination mapping (Figure 2D), is also shown. (B) aux1-116 is allelic to aux1-7. Complementation test showing mean normalized primary root length (±SE; n ≥ 13) of Col-0 wild-type (AUX1/AUX1), aux1-7/aux1-7, aux1-116/aux1-116, and aux1-7/aux1-116 seedlings grown under yellow-filtered light at 22° for 4 d on unsupplemented medium, followed by 4 d on medium supplemented with 10 µM ABA. Asterisks indicate that the mutant normalized root lengths were significantly longer than wild-type normalized root lengths on ABA (**P ≤ 0.001) in two-tailed t tests assuming unequal variance.