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. 2014 May 23;4(7):1319–1326. doi: 10.1534/g3.114.011668

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Copyright © 2014 Ramey and Sclafani

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pre-sensor 1 beta-hairpin structure and growth of mutants. (A) Conservation of the PS1-hp amino acids between Sulfolobus solfataricus Mcm and Saccharomyces cerevisiae Mcm2-7p. (B) Structure prediction of scMcm4p using Phyre software using the ssoMcm structure as a template. Alignment of the ssoMcm(cyan) and scMcm4p (orange) using pymol software, PS1-hp is indicated with an arrow. (C) Structure prediction of scMcm5p using Phyre software using the ssoMcm structure as a template. Alignment of the ssoMcm(cyan) and scMcm5p (magenta) using pymol software, PS1-hp indicated with an arrow. (D) Serial dilution analysis of growth indicates no growth defect of the Mcm4p PS1-hp mutant compared with wild type (Mcm4p-HA). Yeast with the PS1-hp mutation in Mcm5p (mcm5-K506A::URA3) has a cold sensitivity phenotype at 18° and a temperature sensitivity phenotype at 37° as indicated by reduced growth compared with the wild type (Mcm5p-HA).