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Co-immunoprecipitation of the Mcm4p and Mcm5p PS1-hp mutants. (A) Immunoprecipitations of Mcm4p-HA, mcm4p-HA K658A, Mcm5p-HA, and mcm5p-HA K506A were performed using an anti-HA antibody or a no antibody negative control. Western blots were probed with anti-HA antibody for Mcm5p, an anti-Mcm2p antibody, and an anti-Ctd1p antibody. Supernatant and pellet fractions are represented by “S” and “P,” respectively. HA, hemagglutinin.
