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. 2014 May 23;4(7):1319–1326. doi: 10.1534/g3.114.011668

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Copyright © 2014 Ramey and Sclafani

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Plasmid stability of Mcm4p and Mcm5p PS1-hp mutants. (A) Wild type or mcm4-HA K658A::TRP1 or (B) wild type or mcm5-HAT K506A::URA3 yeast strains were transformed with either pDK243 (1 origin of replication) or pDK368-7 (eight origins of replication), and plasmid stability was analyzed. Plasmid loss rates are shown in % loss per generation. The increase in stability with the addition of seven origins of replication is presented as fold increase in stability compared with one origin of replication.