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. 2014 May 23;4(7):1319–1326. doi: 10.1534/g3.114.011668

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Copyright © 2014 Ramey and Sclafani

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chromatin immunoprecipitation (ChIP) analysis of Mcm4p and Mcm5p PS1-hp mutant proteins at origins of replication. (A) ChIP analysis of wild-type (Mcm4-HA) and PS1-hp mutant of Mcm4p (mcm4-K658A-HA) from G1-arrested cultures. Log-phase cultures were arrested in G1 with the addition of alpha factor for 3 hr and an anti-HA antibody was used for ChIP. Chromatin associated DNA was analyzed using quantitative polymerase chain reaction using primers specific to origins of DNA replication. (B) Similar ChIP analysis of wild type (Mcm5-HA) and PS1-hp mutant Mcm5p (mcm5-K506A-HA) strains.