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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Neuroscience. 2005;131(4):871–876. doi: 10.1016/j.neuroscience.2004.10.013

Fig. 3.

Fig. 3

Functional importance of the NPY polymorphisms identified in the 2nd intron, 3rd intron, and 3′UTR. The Luc constructs were transiently transfected into SK-N-SH cells, and the resulting effect on luciferase expression was subsequently determined. SV40, luc+, and poly(A) denote the SV40 promoter, the luciferase gene, and the SV40 late poly(A) signal, respectively. The polymorphisms, located at +4666, +6871, and +7010, are noted in addition to the relative insertion site of each fragment into the pGL-3 Promoter vector. The activity of each construct was normalized to the internal control plasmid, pRL-CMV, and was expressed as fold change compared with the activity of the pGL-3 Promoter vector, which was designated as 1. The bars and fold change show the mean±S.E.M. of the results from seven independent transfection experiments performed in triplicate, using two different plasmid preparations. The significance of differences in the resulting mean values within and between multiple constructs was analyzed using ANOVA. An asterisk denotes a significant reduction in luc expression.