Table 1.
Real-time PCR | |
NPY-1F | 5′-agatactactccgctctgcga-3′ |
NPY-1R | 5′-ggcattttctgtgctttctct-3′ |
GAPDH-F | 5′-cagtcaaggctgagaatggga-3′ |
GAPDH-R | 5′-gggatctcgctcctggaag-3′ |
Vector construction | |
Luc-1F | 5′-cggatccaactttgacttccaacag-3′ |
Luc-1R | 5′-cggatccatcaactctggaaaaac-3′ |
Luc-2F | 5′-cggatccaagtactcccttagagactg-3′ |
Luc-2R | 5′-cggatcctaaaacacaagaggcaaa-3′ |
Luc-3F | 5′-ctctagaatgaaacttgctctcctgactt-3′ |
Luc-3R | 5′-atctagatagtcacaccaggtgttcagtc-3′ |
Luc-4R | 5′-gacgatagtcatgccccgcg-3′ |