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. 2015 Jan 6;8:5. doi: 10.1186/s13071-014-0620-5

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© Abe et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Migration of peritoneal cells to the site of infection. Peritoneal cells (PEC) were obtained from CCR5^−/− mice and C57BL/6 mice (wild-type, WT) at 0, 5 and 10 days post-infection (dpi) with 1 × 10⁶ N. caninum tachyzoites (0 dpi, N = 3; 5 dpi, N = 9 from two independent experiments; 10 dpi, N = 5). The cells were subjected to flow cytometry to determine the absolute number of monocytes/macrophages (A), dendritic cells (B), neutrophils (C), NK cells (D), NKT cells (E) and T cells (F). Cell number per individual (symbols) and mean levels (horizontal lines) are indicated. Data were analyzed by a student’s t-test and compared with values taken on the same day post-infection. *P < 0.05.