Table 1.
Infection rates and number of CD11b + , CD11c + , or CD3 + cells infected with N. caninum -expressing GFP
Infection rates (%) | Infected cell number (×10 4 ) | ||
---|---|---|---|
CD11b+ cells | WT | 2.03 ± 0.70, P = 0.255 | 9.68 ± 3.05, P = 0.73 |
CCR5−/− | 3.08 ± 1.93 | 9.09 ± 2.65 | |
CD11c+ cells | WT | 3.81 ± 1.01, P = 0.534 | 3.79 ± 1.27, P = 0.19 |
CCR5−/− | 4.23 ± 1.23 | 2.85 ± 1.07 | |
CD3+ cells | WT | 0.30 ± 0.18, P = 0.131 | 0.55 ± 0.22, P = 0.45 |
CCR5−/− | 0.80 ± 0.66 | 0.67 ± 0.31 |
Peritoneal cells were obtained from CCR5−/− and C57BL/6 mice (WT) at 5 days after infection with 1 × 106 N. caninum tachyzoites expressing GFP (N = 6). Cells were then subjected to flow cytometry to determine the infection rate and absolute number of monocytes/macrophages (CD11b+), dendritic cells (CD11c+) and T cells (CD3+) based on GFP+ cells. Data were analyzed by a student’s t-test. GFP: green fluorescent protein.