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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Arthritis Rheumatol. 2015 Jan;67(1):86–95. doi: 10.1002/art.38871

Figure 4. Priming effects of TNFα is dependent on sustained NF-κB activity.

Figure 4

(A), A time course (0-72 hours) stimulation of FLS with TNFα (10 ng/ml) was performed and chromatin immunoprecipitation was done with anti-p65 antibody. The enrichment of immunoprecipitated p65 at the CXCL10 promoter is shown as the percentage of input. Results are representative of three independent experiments. (B), FLS were cultured for 3 days in the presence or absence of TNFα (10 ng/ml), which was added on the first day of culture and was not replenished. On day 3, DMSO or IKK inhibitor II (iIKK II, 50μM) was added for 30 minutes and then cells were stimulated for 3 hours with IFNβ (1,000 U/ml) or IFNγ (100 U/ml). The expression of CXCL10/IP10 mRNA was measured by qPCR. Values are the mean ±SEM and were normalized to mRNA for GAPDH. *= p<0.05, by one-tailed paired Student t test.