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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Nature. 2015 Mar 18;520(7545):57–62. doi: 10.1038/nature14344

Figure 1. Identification of SLC7A11as a target of p53.

Figure 1

a, Western blot and RT–PCR for tet-on p53 stable line cells treated with doxycycline. VIN, vinculin. b, Schematic diagram of p53 binding location and sequence on human SLC7A11 gene. Identified p53 binding sequence was compared with consensus sequence (R, A/G; W, A/T; Y, C/T; nucleotides C and G in red are essential for p53 binding). TSS, transcription start site. Facing arrows indicate primers for generating probes in c and PCR in d. c, EMSA was performed with indicated components. The double plus sign represents that more competition cold probes were added compared to the single plus sign (200-fold versus 100-fold to radioactive-labelled hot probes). d, ChIP assay was carried out in U2OS cells. e, U2OS cells with p53 knockdown were treated with nutlin and analysed by western blot. All data are representative of three independent experiments.