Representative photomicrographs of Gly-IR staining in the MNTB from normal hearing (A,B) and 14-day deaf (C,D) rats. B and D are enlargements of regions from A and C. Many of the glycine-immunolabeled principal cells of the MNTB from normal haring animals (A,B) are surrounded by Gly-IR puncta. Following 14 days of deafness the number of glycine immunoreactive axosomatic puncta significantly decreases. Scale bars = 30 μm in C (applies to A,C); 15 μm in D (applies to B,D).