Figure 6.

Double labeling immunofluorescence showing vGluT1 (green) and vGluT2 (red) in the hearing rat PVCN granule cell domain (A-A‴) and in the DCN (B-B‴). In the PVCN-Gr, many terminals are labeled for both vGluTs as indicated in yellow (arrows in A′-A‴). Within the DCN, the majority of vGluT1 and vGluT2 terminals show complementary distribution, however glutamatergic axon terminals coexpressing both isoforms are frequently seen in the DCN-F and the DCN-D layers (arrows in B′-B‴). PVCN-Gr; PVCN granule cell domain, DCN-M; molecular layer of DCN, DCN-F; fusiform cell layer of DCN, DCN-D; deep core layer of DCN. Scale bars shown in A‴ and B‴ valid for A′-A‴ and B′-B‴ respectively.