Figure 2.

Fate of the CarH 5′-deoxyadenosyl group upon photolysis. Shown are A₂₆₀ traces from LC of different light-exposed CarH and free AdoCbl samples. CarH photolyzed under aerobic (red) or anaerobic (brown) conditions yields only a single nucleoside product with a retention time and a molecular mass different from those of nucleoside products derived from free AdoCbl photolyzed under aerobic (black) or anaerobic (green) conditions. Mass-to-charge ratios of peaks, as determined by an in-line mass spectrometer, are indicated. Peaks denoted with an asterisk are cobalamin species, as indicated by additional absorbance at 350 and 520 nm.