Fig 1. Confirmation of the C. Cayetanensis mt genome sequence.

A) Overlapping PCR fragments used to confirm the C. cayetanenesis mt genome sequence and concatemeric structure. PCR fragments are shown with dotted lines. Forward sequence reads are shown in blue or orange and reverse reads are shown in red or light blue. Terminal grey bands indicate identical sequence regions. B) A 659 bp contig constructed using overlapping junction PCR fragment sequences (see Table 2) is represented in gray. Diagonal red bars represent portions of the junction PCR product mapping to the ends of the complete C. cayetanensis mt genome. C) WGS reads spanning the junction region in the initial mt assembly. The vertical red lines mark the tail:head junction.