Table 2. PCR primer sets and amplicons.
Full length mt genome PCR | |||
Amplicons | Forward Primer | Reverse Primer | Amplicon size (bp) |
A | 2F-Begin | 2R | 1393 |
B | 3Fb | 3Rb | 1609 |
C | 4Fb | 4Rb | 1984 |
D | 5F | 5R | 901 |
E | 4B-F | 4B-R | 1022 |
F | 1F | 1R | 1507 |
G | 2Fb | End5-R | 458 |
Junction PCR | |||
J1 | End1-F | End6-R | 634 |
J2 | 2Fb | End5-R | 458 |
J3 | End2-F | End6-R | 603 |
J4 | End3-F | End6-R | 541 |
J5 | End4-F | End6-R | 535 |