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. 2015 Jun 4;10(6):e0128712. doi: 10.1371/journal.pone.0128712

Table 1. The oligonucleotides used for EEEV full genome sequencing.

Name Use Sequence Tm (C)
EEE 40 FWD RT-PCR and sequencing GGCAACCACCCTATTTCCACCTA 70
EEE 626 FWD Sequencing GGCGCCTACCCTACATACA 60
EEE 1087 FWD Sequencing ATGACCGGGATACTGGCGACTGAC 76
EEE 1349 REV Sequencing GGCGGGCACCTTCTTAATAGTTTG 72
EEE 2059 REV RT-PCR and sequencing GCATCCCCTTTCTTCACGCACTTC 74
EEE 1824 FWD RT-PCR and sequencing CGCGCAGGACGATACAA 54
EEE 2586 FWD Sequencing CTCGGCGATGCACTAAGAC 60
EEE 3030 FWD Sequencing CATGGCGAAAATACTTGAGAC 60
EEE 3438 FWD Sequencing ATAACCCGCTAATAAATGT 50
EEE 4035 REV RT-PCR and sequencing TTATGTCGCCGCGCACCACTCTAT 74
EEE 3836 FWD RT-PCR and sequencing GCAGTCGCCCGCTCATTCA 62
EEE 4619 FWD Sequencing CCGAGGGCAAGGTGTATT 56
EEE 4956 REV Sequencing GCCGGGGGTACAGTGCCAGAGA 74
EEE 5102 FWD Sequencing TGCAAGAATCCCCAGCCCTCCAT 72
EEE 5742 REV RT-PCR and sequencing GTTTGCATTGCCGCGTAGATTTTT 68
EEE 5568 FWD RT-PCR and sequencing GAGCCGCAGCGCAGACACGAT 70
EEE 6252 FWD Sequencing ATTTGCGGCCTGAGATACG 58
EEE 6562 REV Sequencing ACATCCCGTTTAAGGTCCATC 62
EEE 7081 FWD Sequencing CGCAGCATTTATCGGCGACGACAA 74
EEE 7776 REV RT-PCR and sequencing CGTTTGGCGGGCGGTCCTG 66
EEE 7509 FWD RT-PCR and sequencing CCATAACCCTCTACGGCTGACCTAAAT 65
EEE 8352 FWD Sequencing AAAGGGGGTTACAGTCAAAGATAC 68
EEE 8666 REV Sequencing GCATGCGCATCCCCTCTGACTTC 74
EEE 9403 REV RT-PCR and sequencing TGGTCCGGGTGCAGGTGTAAAATC 74
EEE 9153 FWD RT-PCR and sequencing CGAGCGGCGCCCAAGTGAAATA 70
EEE 9536 FWD Sequencing CCGGAGAAGGGTTGGAGT 58
EEE 9877 REV Sequencing GGATAAGCGTCTGCATCCAG 64
EEE 10466 FWD Sequencing ATGGTGAAACTCCCGCGAAAATAG 70
EEE 11183 REV Sequencing TCGCCGACGTAAAGGATTC 58
T25 REV RT-PCR and sequencing T(25)V 78