Table 1. The oligonucleotides used for EEEV full genome sequencing.
Name | Use | Sequence | Tm (C) |
---|---|---|---|
EEE 40 FWD | RT-PCR and sequencing | GGCAACCACCCTATTTCCACCTA | 70 |
EEE 626 FWD | Sequencing | GGCGCCTACCCTACATACA | 60 |
EEE 1087 FWD | Sequencing | ATGACCGGGATACTGGCGACTGAC | 76 |
EEE 1349 REV | Sequencing | GGCGGGCACCTTCTTAATAGTTTG | 72 |
EEE 2059 REV | RT-PCR and sequencing | GCATCCCCTTTCTTCACGCACTTC | 74 |
EEE 1824 FWD | RT-PCR and sequencing | CGCGCAGGACGATACAA | 54 |
EEE 2586 FWD | Sequencing | CTCGGCGATGCACTAAGAC | 60 |
EEE 3030 FWD | Sequencing | CATGGCGAAAATACTTGAGAC | 60 |
EEE 3438 FWD | Sequencing | ATAACCCGCTAATAAATGT | 50 |
EEE 4035 REV | RT-PCR and sequencing | TTATGTCGCCGCGCACCACTCTAT | 74 |
EEE 3836 FWD | RT-PCR and sequencing | GCAGTCGCCCGCTCATTCA | 62 |
EEE 4619 FWD | Sequencing | CCGAGGGCAAGGTGTATT | 56 |
EEE 4956 REV | Sequencing | GCCGGGGGTACAGTGCCAGAGA | 74 |
EEE 5102 FWD | Sequencing | TGCAAGAATCCCCAGCCCTCCAT | 72 |
EEE 5742 REV | RT-PCR and sequencing | GTTTGCATTGCCGCGTAGATTTTT | 68 |
EEE 5568 FWD | RT-PCR and sequencing | GAGCCGCAGCGCAGACACGAT | 70 |
EEE 6252 FWD | Sequencing | ATTTGCGGCCTGAGATACG | 58 |
EEE 6562 REV | Sequencing | ACATCCCGTTTAAGGTCCATC | 62 |
EEE 7081 FWD | Sequencing | CGCAGCATTTATCGGCGACGACAA | 74 |
EEE 7776 REV | RT-PCR and sequencing | CGTTTGGCGGGCGGTCCTG | 66 |
EEE 7509 FWD | RT-PCR and sequencing | CCATAACCCTCTACGGCTGACCTAAAT | 65 |
EEE 8352 FWD | Sequencing | AAAGGGGGTTACAGTCAAAGATAC | 68 |
EEE 8666 REV | Sequencing | GCATGCGCATCCCCTCTGACTTC | 74 |
EEE 9403 REV | RT-PCR and sequencing | TGGTCCGGGTGCAGGTGTAAAATC | 74 |
EEE 9153 FWD | RT-PCR and sequencing | CGAGCGGCGCCCAAGTGAAATA | 70 |
EEE 9536 FWD | Sequencing | CCGGAGAAGGGTTGGAGT | 58 |
EEE 9877 REV | Sequencing | GGATAAGCGTCTGCATCCAG | 64 |
EEE 10466 FWD | Sequencing | ATGGTGAAACTCCCGCGAAAATAG | 70 |
EEE 11183 REV | Sequencing | TCGCCGACGTAAAGGATTC | 58 |
T25 REV | RT-PCR and sequencing | T(25)V | 78 |