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. 2015 Jun 4;10(6):e0128551. doi: 10.1371/journal.pone.0128551

Fig 1. The expression of ANKRD49 in a panel of mouse tissues and its temporal expression in developing mouse testes.

Fig 1

(A) Alignment of the amino acid sequence of mouse ANKRD49 (AAH19777.1) and human (NP_060174.2), Macaca mulatta (AFH27401.1), Pan troglodytes (JAA43031.1), Bos_aurus (NP_001014965.1), Canis_lupus (XP_005633413.1) and rat (AAI61982.1). The alignment was performed by DNAMAN (Lynnon, Quebec, Canada). Homology levels are highlighted in different colours. Black: 100%; Pink: 75%; Blue: 50%. (B) Phylogenetic tree analysis of ANKRD49 and ankyrin repeat family proteins. Numbers indicate branch length. (C) The tissue distribution of ANKRD49 mRNA in adult mice was analysed by quantitative RT-PCR. (D) The expression pattern of ANKRD49 in a panel of tissues from adult mice was determined by Western blot. (E and F) The mRNA and protein levels of ANKRD49 in the testes of mice one to eight weeks old were detected by qRT-PCR and Western blot. GAPDH served as a loading control. Each assay was repeated three times with similar results. W is the abbreviation for ‘‘week.” Each number represents the age of the mice.