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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Breast Cancer Res Treat. 2015 Mar 6;150(2):309–320. doi: 10.1007/s10549-015-3324-4

Fig. 2.

Fig. 2

Calcium concentrations and N/P ratios modulate transfection efficiency of Ca-TAT complexes to PyVmT mammary carcinoma cells. a Mammary fibroblasts (Fib), Raw 264.7 macrophages (Mϕ) or PyVmT mammary carcinoma cells, were transfected with Ca-TAT peptides complexed to MSCV-luciferase plasmids at the indicated CaCl2 concentrations and N/P ratios. Luciferase activity was measured 48 h post-transfection. RLU relative light units. b Ca-TAT peptides were complexed to control siRNA (Con) or CCL2 siRNAs (mCCL2si), and transfected into the indicated cell lines. CCL2 expression was measured in parental (Par) or transfected cells by ELISA 48 h later. Statistical analysis was performed by One-Way ANOVA, followed by Bonferroni post-hoc comparisons. Statistical significance was determined by p value < 0.05. *p < 0.001,***p < 0.05, ****p > 0.05. Values are shown as Mean ± SEM