Fig. 3.

Ca-TAT/siRNA complexes selectively target CCL2 gene expression in mammary tumor tissues cultured ex vivo. a Left panel Diagram of PyVmT mammary tumors cultured in suspension ex vivo. Ca-TAT complexes are injected directly into the tumor tissue. Right panel representative image of a tumor sample shown in an overhead view. b Flow cytometry analysis was performed to measure overall CCL2 expression in normal mammary tissues or PyVmT tumors injected with phosphate buffered saline (PBS vehicle control), or Ca-TAT peptides complexed to control siRNA (Con) or CCL2 siRNA (mCCL2si). CCL2 expression levels were normalized to secondary antibody only control, and are relative to whole cell population in either the tumor or normal group. N = 6 per group. Statistical analysis was performed using One-Way ANOVA with Bonferroni post-hoc comparison. Statistical significance was determined by p value < 0.05. ***p < 0.05, ****p > 0.05. Values are shown as Mean ± SEM