Skip to main content
. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Breast Cancer Res Treat. 2015 Mar 6;150(2):309–320. doi: 10.1007/s10549-015-3324-4

Fig. 6.

Fig. 6

CCL2 gene silencing in PyVmT mammary carcinoma cells leads to increased necrosis and autophagy. PyVmT cells were transfected with Ca-TAT peptides complexed to control (Con) or CCL2 siRNAs (mCCL2si). 48 h later, transfected cells or parental cells (Par) were analyzed for the following: a Propidium iodide (PI) staining by flow cytometry. b Autophagosome formation by uptake of Cyto-ID® green dye. LC3B expression was quantified by Image J. Levels were normalized to DAPI stain. Statistical analysis was performed using One-Way ANOVA with Bonferroni post-hoc comparisons. Significance was determined by p < 0.05. ***p < 0.05, ****p > 0.05. For (A), ***p < 0.05 versus Control siRNA group. Values are shown as Mean ± SEM